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- Rooting Studies on Black Pepper (Piper nigrum Linn.) Stem Cuttings
Rooting Studies on Black Pepper (Piper nigrum Linn.) Stem Cuttings
Thesis Abstract:
Experiments were conducted to determine the rooting response of runner and lateral fruiting stem cuttings to synthetic growth regulators, to ascertain differences in endogenous rooting cofactors between the two types of planting materials, and to examine the anatomical structure of the stem cuttings in relations to adventitious root formation.
Lateral fruiting stems failed to root even when treated with four synthetic growth regulators, namely, IBA ( Indole butyric acid), NAA (Napthalene acetic acid), Alar (Succinic acid 2-2 Dimethyl hydroxide) and Quercitin. In contrast, runner cuttings successfully rooted with or without application of the four substances.
No differences were observed in the levels of endogenous promoters and inhibitors in the two types of cuttings. Cofactor 4 was found to be the major root-promoting substance in both types of planting materials.
Root primordial originated from the cascular rays in the interfascicular region between the vascular bundles in both types of cuttings.
Morphological changes likely accounted for the major differences in the root formation of the two types of cuttings. Cell walls of the vascular rays of fruiting stem cuttings were more lignified than those of runner stems, suggesting that the poor rooting capacity of the former was due to much less activity of the vascular ray cells during the dedifferentiation process.
Successful rooting of lateral fruiting stems with slanted cut at the node indicated that aside from anatomical factors, other effects were involved in root regeneration.