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Influence of Selected Growth Regulators and Organic Additives on Culture In Vitro of Papaya (Carica papaya L.) Ovary Tissue
Thesis Abstract:
Experiments were conducted to determine the effect of some growth regulators and organic additives on callus formation and differentiation of papaya ovary tissue culture in vitro.
Among the growth regulators tested, indole butyric acid (IBA), gibberellic acid (GA) and benzyl adenine (BA) were found to enhance the formulation and growth of papaya callus. At very low level, 2, 4-dichlorophenoxy (2,4-D) also promoted the development of papaya callus.
Among the plant extracts tested, coconut water proved to be better than yeast extract, edamin and papaya juice. However, the treatment combination of coconut water, tyrosine and 2,4-D surpassed all other combinations. In one instance, this combination even stimulated root formation from papaya callus.
The basic regulatory mechanism involving a balance between auxin and cytokinin did not seem applicable for root or shoot initiation in papaya tissue. Inositol, potassium and phosphate levels and also change in different nutrient compounds in some media proved ineffective with regard to this morphogenesis problem.
Starch as an energy source for organ formation was also tested, but its effect was not demonstrated in this study. Starch combined with BA greatly increased papaya callus development. However, certain inhibitors in papaya tissue could have interfered with the regulatory system or enzyme activities, thus inhibiting the formation of roots or shoots from papaya tissue cultured in vitro.