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Isolation, Serotyping and Pathogenicity Study of Salmonella spp. Lignieres in Cattle (Bos Indicus Linn.) Slaughtered in Laguna and Batangas
Thesis Abstract:
The study aimed to identify serotypes and determine the pathogenicity of Salmonella spp. isolated from 149 apparently healthy cattle slaughtered in four selected abattoirs in Laguna and Batangas to compare the recovery rate in different culture media combinations. Mesenteric lymph nodes from each cattle were collected and cultured in four selective enrichment-selective plating media combinations, namely: Rappaport-Vassiliadis broth-Xylose Lysine Desoxycholate agar (RV-XLD), Rappaport-Vassiliadis broth-Brilliant Green Agar (RV-BGA), Tetrathionate Brilliant Green broth-Xylose Lysine Desoxycholate agar (TBG-XLD) and Tetrathionate Brilliant Green broth-Brilliant Green Agar (TBG-BGA). The proportion of slaughtered cattle positive for Salmonella was 17.4 percent, highest in Sta. Cruz (30%) and lowest in San Pablo abattoirs (11.1%). The difference was statistically significant (p < .05).
The RV-XLD media combination had the highest recovery rate of 12.8 percent followed by RV-BGA (9.4%), TBG-BGA (8.7%), and TBG-XLD (6.0%). There was a significant difference in recovery rate among these media combinations (p < .05). Seven serotypes of Salmonella belonging to serogroups B, C1, D and E, and one untypable isolate were identified. These were S. derby, S. anatum, S. saint-paul, S. javiana, S. weltevreden, S. lexington, and S. thompson. S. derby accounted for 30.76 percent (8/26) of total isolates. By the infant mouse test, 84.62 percent were found positive for enterotoxin. None of 26 Salmonella isolates were positive for invasiveness using the Sereny test. The results indicated that slaughtered cattle sampled in this study served as sources of enterotoxin-producing Salmonella in humans. Hence, surveillance of Salmonella and other food-borne diseases in slaughterhouses is needed to prevent them from reaching the consuming public.