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Insecticidal Activity of Rocaglamide and Immunity Inhibition of Crocidolomia Pavonana (Fabricus) to Eriborus Argenteopilosus (Cameron)
Abstract:
Rocaglamide, the major active constituent of Aglaia odorata extract showed strong activity against some insect species. Rocaglamide and its derivatives were reported to inhibit growth and proliferation of specific cancer cells. Some rocaglamide derivatives inhibited protein synthesis in cell culture. Although rocaglamide has been tested on some insect pest species, its effects on pest natural enemies and host-parasitoid interaction have never been reported. Field and laboratory research had been conducted to (1) evaluate the potency of rocaglamide against second instar larvae of Crocidolomia pavonana, (2) study the toxicity of rocaglamide on female adults of Eriborus argenteopilosus parasitoid, (3) study the effect of rocaglamide on parasitization and defense reaction of C. pavonana to the immature E. argenteopilosus parasitoid, (4) study the effect of rocaglamide on foraging behavior of E. argenteopilosus female, (5) study the phytotoxicity of rocaglamide and fraction of A. odorata branch extract on broccoli and soybean. Field experiment had been conducted to evaluate compatibility branch extract of A. odorata and E. argenteopilosus parasitoid.
Rocaglamide at the highest concentration (300 ppm) by topical application (1 μl/insect) was relatively nontoxic to the female of E. argenteopilosus. Rocaglamide treatment on host larvae did not reduce parasitization rate, eventhough it could suppress encapsulation rate of parasitoid egg and larvae. The treatment of rocaglamide reduced total hemocyte counts, hemolymph protein content, and altered profile hemolymph protein of fourth instar larvae of C. pavonana. This may explain the reduced capacity of C. pavonana larvae to encapsulate parasitoid egg and larvae. Rocaglamide at 300 ppm had no phytotoxic effect on broccoli and soybean leaves. In contrast, ethyl acetate fraction from separator channel of A. odorata extract and dichloromethane, dichloromethane-isoprophanol fraction from vacuum liquid chromatography at concentration 0.43 percent showed strong phytotoxicity on broccoli and soybean leaves. Field evaluation showed that spraying of 0.28 percent A. odorata extract did not influence population of C. pavonana larvae and egg cluster as well as its parasitoid E. argenteopilosus. Because of phytotoxicity properties of active fraction of A. odorata extract, using this extract in pest control must be evaluated. Results of the research showed that rocaglamide has potency to increase probability of parasitoid survival, such that rocaglamide has a good potential to be combined with biological control measures using parasitoid in cabbage pest management system.