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Asian Journal of Agriculture and Development (AJAD) - Call for papers!

Selection, characterization, and infectivity of entomopathogenic fungi against the spider mite (Tetranychus kanzawai (Kishida) (Tetranychidae: Acarina).

(Indonesia), Doctor of Philosophy in Entomology (University of the Philippines Los Baños)

Dissertation Abstract:

Six isolates of Beauveria bassiana (Bb1–Bb6), seven isolates of Metarhizium anisopliae (Ma1–Ma7), and one isolate of Paecilomyces lilacinus (P1) from Philippines and Indonesia were evaluated for their infectivity to Tetranychus kanzawai (Kishida). The selected three isolates each of Bb and Ma were pathogenic to mite with LC50 value of 1.2 × 103 to 2.4 × 103, 5.0 × 102 to 1.3 × 103 for Ma after days post infection under laboratory condition. When 108 concentrations of the seven isolates were sprayed to papaya seedlings in the greenhouse, LT50 values ranged from 3.0 to 4.2 days for Ma and 3.98 to 5.48 days for Bb. P1 was not infective to T. kanzawai compared with the other two fungal pathogens.
 
Characterization using morphological and molecular analyses showed that selected Ma, Bb, and P1 entomopathogenic isolates virulent against T. kanzawai belong to B. bassiana, M. anisopliae, and P. lilacinus species, respectively. Its DNA sequence analyses also indicated variation within Beauveria and Metarhizium isolates.