Search Filter
- Publications
- Abstract of Theses and Dissertations
- Database
- Morphological and molecular characterization of Hoya mindorensis...
Morphological and molecular characterization of Hoya mindorensis Schlechter
Thesis Abstract:
Hoyas are increasingly gaining popularity as ornamental crops worldwide; however, they are still considered underutilized species and many were just recently cultivated from its natural habitat. The knowledge on identification of the different Hoya species is still fragmentary. Hoya mindorensis is an endemic species of the Philippines. The H. mindorensis collection at the Institute of Plant Breeding (IPB) varies in flower size, flaring of outer corona lobes, color, hair density on corolla, and leaf shape. This study aimed to develop a preliminary descriptor list and to
characterize H. mindorensis collection using morphological and molecular markers. It also aimed to develop appropriate DNA extraction method and optimal PCR protocol for H. mindorensis.
Sixteen accessions of H. mindorensis from IPB collection were characterized; however, only 11 were morphologically characterized due to non-flowering of five accessions. DNA extraction protocol for H. mindorensis and optimalization of PCR amplification using 12 SSR primers from Amsonia kearneyana was developed.
Results proved that the different clusters in H. mindorensis accessions have morphological similarities in leaf texture, top corona margin, and upper corona lobe apex color. Accession #05, 12, and 13 were closely related and can be separated into a different subgroup based on the morphological data; however, accession #12 is so different from accession #05 and 13 based on molecular data.
A modified Angeles et al. (2005) protocol was successful in obtaining DNA products. With this protocol, the amount of β-mercaptoethanol concentration was modified and the use of SDS was omitted. Four compatible SSR markers were successfully transferred from A. kearneyana to H. mindorensis. The total polymorphic bands obtained were as high as 73.1 percent. The morphological characterization suggested that the accessions can be clustered into four clusters, while according to molecular characterization, they can be clustered into five clusters. Both characterizations resulted in different population intra-groups.
Based on the overall morphological performance, four accessions (#05, 09, 11, and 12) can be mass propagated for commercialization, while some accessions (#02, 03, and 10) have great potential as parent materials for future improvement in H. mindorensis and other Hoya species.